Coconut (Cocos nucifera L.) is one of the most important fruit trees in Bangladesh. This tree is susceptible to various pathogens. Among them, a fungus was consistently isolated from gray leaf spot symptom in coconut. This study aimed to isolate, characterize, and find the management strategies of the causal fungus of gray leaf spot disease in coconut. Both morphological and molecular characters identified the pathogen as Pestalotiopsis sp. for the first time in Bangladesh. Artificial inoculation of this fungus showed symptoms similar to those previously observed in the field. Cross-inoculation test suggests that Pestalotiopsis sp. has a wide host range. The infection process of Pestalotiopsis sp. started at 2 h after inoculation (hai) with the formation of germ tube followed by the formation of infection hyphae, which penetrated directly into the host at 6 hai. Gray leaf spot symptom was developed at 120 hai. Numerous conidia developed from the acervuli at 168 hai. These conidia acted as the source of inocula for secondary infection. The optimum temperature for the growth of Pestalotiopsis sp. was 25°C, however, the growth of Pestalotiopsis sp. ceased at 15°C and 35°C. This pathogen was completely inhibited by Autostin 50 WDG (carbendazim) at 100 ppm. Trichoderma viride (Pb-7) was found as the potential biocontrol agent against Pestalotiopsis sp. These findings could contribute to describing the disease cycle and epidemiology of Pestalotiopsis sp. that would ultimately require to undertake effective control measures against this pathogen.